Home
Class 12
BIOLOGY
Insertional inactivation is related to: ...

Insertional inactivation is related to:
(a) Microinjection
(b) Gene gun
(c) Gel electrophoresis
(d) Selection of recombinants

A

Microinjection

B

Gene gun

C

Gel electrophoresis

D

Selection of recombinants

Text Solution

Verified by Experts

The correct Answer is:
D
Promotional Banner

Similar Questions

Explore conceptually related problems

Emasculation is related to (a) Pure line (b) Mass selection (c) Clonal selection (d) Hybridisation

How is insertional inactivation of an enzyme used as a selectable marker to differentiate recombinants from non-recombinants?

Assertion: The inactivation of any vector gene due to insertion of desired gene called as insertional inactivation Reason :Some time restriction sites are also present in between the gene sequence of vector

In insertional inactivation of beta -galactosidase gene, the bacteria in white colonies have

In the process of insertional inactivation 1) a recombinant DNA is inserted within the coding sequence of enzyme β -galactosidase, resulting in inactivation of the enzyme 2) a recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid 3) a recombinat DNA is inserted within the recongnition site for EcoRl 4) none of these

Pick out the correct statement from the following in relation to gel electrophoresis

Some of the step involved in the production of humulin are given below. Arrange them in the correct sequence and select the correct option (i) Synthesis of gene (DNA) for human insulin artifically (ii) Culturing recombinant E.coli in bioreactors (iii) Purification of humulin (iv) Insertion of human insulin gene into plasmid (vi) Etraction of recombinant gene product from E.coli.

Some of the steps involved in production of humulin are given below. Choose the correct sequence (i) Synthesis of insulin gene artificially (ii) Culturing recombinant E. coli in bioreactors (iii) Purification of humulin (iv) Insertion and human insulin gene into plasmid (v) Introduction of recombinant plasmid into E. coli (vi) Extraction of recombinant gene product from E. coli.

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

Explain the role(s) of the following Biotechnology: a) Restriction endonuclease Gel-electrophoresis c) Selectable markers in pBR322.