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Which of the following statements are fa...

Which of the following statements are false regarding 'Hershey and Chase' experiment, proving DNA is genetic material ?
(i) They made use of rats.
(ii) Radioactivity was seen in supernatant for the medium that was infected by phase radiolabelled with `.^(32)P`.
(iii) Used radioisotope to label sulphur with `.^(35)S` in sulphur- containing amino acids.
(iv) Radioactive phages were allowed to attach to E. coli bacteria.

A

(ii) and (iii) only

B

(iii) and (iv)

C

(ii) , (iii) and (iv) only

D

(i) and (ii)

Text Solution

AI Generated Solution

The correct Answer is:
To determine which statements are false regarding the Hershey and Chase experiment that proved DNA is the genetic material, we will analyze each statement one by one. ### Step-by-Step Solution: 1. **Statement (i)**: "They made use of rats." - **Analysis**: This statement is false. The Hershey and Chase experiment did not involve rats; instead, they used E. coli bacteria and T2 bacteriophage. Rats were used in the Griffith experiment, not in Hershey and Chase's work. 2. **Statement (ii)**: "Radioactivity was seen in supernatant for the medium that was infected by phage radiolabelled with .^(32)P." - **Analysis**: This statement is also false. When E. coli was infected with the phage that was labeled with radioactive phosphorus (.^(32)P), the radioactivity was found inside the bacterial cells, not in the supernatant. The supernatant contained the protein coat of the phage, which did not carry the radioactive phosphorus. 3. **Statement (iii)**: "Used radioisotope to label sulphur with .^(35)S in sulphur-containing amino acids." - **Analysis**: This statement is true. The Hershey and Chase experiment correctly used .^(35)S to label the proteins of the phage, as sulfur is found in amino acids like cysteine and methionine. 4. **Statement (iv)**: "Radioactive phages were allowed to attach to E. coli bacteria." - **Analysis**: This statement is true. The experiment involved allowing radioactive phages to attach to E. coli, which was a crucial part of demonstrating that DNA, not protein, was the genetic material. ### Conclusion: The false statements regarding the Hershey and Chase experiment are: - (i) They made use of rats. - (ii) Radioactivity was seen in supernatant for the medium that was infected by phage radiolabelled with .^(32)P. ### Final Answer: The false statements are (i) and (ii). ---
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