Home
Class 12
BIOLOGY
If alien DNA is introduced using Pst I i...

If alien DNA is introduced using Pst I in pBR322 then transformant will grow on :

A

Ampicillin

B

Tetracyline

C

Both (A) and (B)

D

None of these

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question of whether the transformant will grow on MPSL (ampicillin) or tetracycline when alien DNA is introduced using Pst I in pBR322, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding pBR322**: - pBR322 is a plasmid vector that contains two antibiotic resistance genes: one for ampicillin (Amp^R) and one for tetracycline (Tet^R). 2. **Identifying Restriction Sites**: - The plasmid has unique restriction sites for various enzymes, including Pst I. The Pst I site is located within the ampicillin resistance gene (Amp^R). 3. **Introducing Alien DNA**: - When alien DNA is introduced into pBR322 using the Pst I restriction enzyme, it will cut the plasmid at the Pst I site. If the alien DNA is inserted into this site, it will disrupt the ampicillin resistance gene. 4. **Effect on Antibiotic Resistance**: - Since the ampicillin resistance gene is disrupted by the insertion of the alien DNA, the transformed cells will lose the ability to grow in the presence of ampicillin. 5. **Growth on Tetracycline**: - The tetracycline resistance gene remains intact because the alien DNA was inserted into the ampicillin resistance gene. Therefore, the transformed cells will retain their ability to grow in the presence of tetracycline. 6. **Conclusion**: - The transformants will grow on tetracycline but not on ampicillin. Thus, the correct answer to the question is that the transformants will grow on tetracycline. ### Final Answer: The transformants will grow on tetracycline. ---
Promotional Banner

Similar Questions

Explore conceptually related problems

In a experiment , in pBR322 an alien DNA is introduced using sal I. The transformant will grow on

In order to insert alien DNA at the site of tetracycline resistance gene in pBR322 the restriction endonuclease used can be :

If the gene of interest is cloned at ECoRI in pBR322, the recombinant E-coli after transformation are

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

If we ligate a foreign, DNA at the Barn HI site of tetracycline resistance gene in pBR322 the recombinant plasmid will:

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reason could be

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reason could be

If we ligate a foreign, DNA at the Barn HI site of tetracycline resistance gene in pBR322 the recombinant plasmid u ill:

If the gene of interest was cloned at Pvu I site in pBR322 ,the recombinant bacteria would exhibit resistance to which drug ?

If gene encoding chain 'A' of human insulin was incorporated at Pst I site of plasmid pBR322, the recombinants