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You discovered a novel eukaryotic organi...

You discovered a novel eukaryotic organism that glows in the dark. You believe this trait is due to a single gene, and you wish to clone the gene. Which of the following strategies is most likely to be successful ?

A

Isolate the genomic DNa from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into bacteria. Screen colonies for the ability to glow in the dark

B

Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark .

C

Isolate the genomic mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into bacteria. Screen colonies for the ability to glow in the dark .

D

Isolate the genomic mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark .

Text Solution

Verified by Experts

The correct Answer is:
D
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