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A bacterium having rDNA formed by using ...

A bacterium having rDNA formed by using pBR322 and EcoRI restriction enzyme is resistant to :

A

Penicillin and ampicillin

B

Ampicillin and tetracycline

C

Tetracycline only

D

Ampicilin only

Text Solution

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The correct Answer is:
To solve the question regarding the resistance of a bacterium with recombinant DNA (rDNA) formed using the pBR322 plasmid and EcoRI restriction enzyme, we can follow these steps: ### Step 1: Understand the Components - **pBR322**: This is a plasmid that contains genes for resistance to two antibiotics: ampicillin and tetracycline. - **EcoRI Restriction Enzyme**: This enzyme is used to cut DNA at specific sites, allowing for the insertion of foreign DNA into the plasmid. ### Step 2: Identify the Resistance Genes - The pBR322 plasmid has: - An **ampicillin resistance gene**: This gene allows bacteria to survive in the presence of ampicillin. - A **tetracycline resistance gene**: This gene allows bacteria to survive in the presence of tetracycline. ### Step 3: Insertion of Gene of Interest - The question states that a gene of interest is inserted into the pBR322 plasmid at the EcoRI site. Importantly, this insertion does not affect the ampicillin or tetracycline resistance genes. ### Step 4: Growth in Selective Media - When the transformed bacterium (with rDNA) is cultured in media containing: - **Ampicillin**: The bacterium will survive because it retains the functional ampicillin resistance gene. - **Tetracycline**: The bacterium will also survive because it retains the functional tetracycline resistance gene. ### Step 5: Conclusion - Since the bacterium can grow in both media containing ampicillin and tetracycline, it is resistant to both antibiotics. ### Final Answer The bacterium having rDNA formed by using pBR322 and EcoRI restriction enzyme is resistant to **both ampicillin and tetracycline**. ---
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