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Select the correct statement about the R...

Select the correct statement about the RNAi method.

A

RNAi strategy was taken up to prevent the infestation of nematodes in the roots maize plants.

B

This method involves the use of a complementary dsRNA molecule that binds to ans prevents translation of the mRNA

C

Only RNA genomes of viruses can be used as a source for the complementary RNA

D

Mobile genetic elelments called transposons can replicate only through a DNA intermediate

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question regarding the correct statement about the RNA interference (RNAi) method, we will analyze each option provided. ### Step-by-Step Solution: 1. **Understanding RNAi**: - RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules. It is a crucial mechanism for regulating gene expression and defending against viral infections in eukaryotic cells. 2. **Analyzing Option 1**: - The first statement claims that "RNAi strategy was taken up to prevent the infestation of nematodes in the roots of maize plant." - This statement is **incorrect** because RNAi is primarily used in the context of certain plants like tobacco to combat nematode infestations, not specifically maize. 3. **Analyzing Option 2**: - The second statement says, "This method involves the use of complementary dsRNA that binds to and prevents the translation of mRNA." - This statement is **correct**. RNAi utilizes double-stranded RNA (dsRNA) that is complementary to the target mRNA, leading to the degradation of the mRNA and preventing its translation. 4. **Analyzing Option 3**: - The third statement states, "Only RNA genomes of viruses can be used as a source of the complementary RNA." - This statement is **incorrect** because RNAi can utilize various sources of RNA, not just viral RNA genomes. It can also involve transposable elements and other RNA sources. 5. **Analyzing Option 4**: - The fourth statement claims, "Mobile genetic elements called transposons can replicate only through a DNA intermediate." - This statement is **incorrect**. Transposons can replicate through both DNA and RNA intermediates, depending on the type of transposon. ### Conclusion: - The only correct statement about the RNAi method is **Option 2**: "This method involves the use of complementary dsRNA that binds to and prevents the translation of mRNA."
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