In glycolysis, ATP is used in which of the following reactions?

Options

A. Conversion of fructose 6- phosphate to fructose 1, 6 - bisphosphate
B. Conversion of 3- phosphoglyceraldehyde is converted to 1, 3- bisphosphoglycerate
C. Conversion of glucose into glucose 6-phosphate
D. Both (A) and (C )

Answer

To solve the question regarding the use of ATP in glycolysis, we can follow these steps:

### Step-by-Step Solution:

1. **Understand Glycolysis**: Glycolysis is a metabolic pathway that converts glucose into pyruvate, producing a small amount of ATP and NADH in the process. It consists of ten enzyme-catalyzed reactions.

2. **Identify ATP-Dependent Reactions**: In glycolysis, ATP is used in the initial steps to phosphorylate glucose and fructose 6-phosphate. Specifically, we need to identify which reactions utilize ATP.

3. **Examine the Given Options**:
- **Option A**: Conversion of fructose 6-phosphate to fructose 1,6-bisphosphate.
- **Option B**: Conversion of 3-phosphoglyceraldehyde to 1,3-bisphosphoglycerate.
- **Option C**: Conversion of glucose to glucose 6-phosphate.
- **Option D**: Both A and C.

4. **Analyze Each Reaction**:
- **For Option A**: The conversion of fructose 6-phosphate to fructose 1,6-bisphosphate involves the enzyme phosphofructokinase, which uses one ATP molecule.
- **For Option B**: The conversion of 3-phosphoglyceraldehyde to 1,3-bisphosphoglycerate does not use ATP; instead, it produces NADH.
- **For Option C**: The conversion of glucose to glucose 6-phosphate involves the enzyme hexokinase and uses one ATP molecule.

5. **Determine the Correct Answer**: Since ATP is used in both the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate (Option A) and glucose to glucose 6-phosphate (Option C), the correct answer is **Option D**: Both A and C.

### Final Answer:
The correct option is **4: Both A and C**.