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Find the correct statement:...

Find the correct statement:

A

Biomacromolecules are found in the acid soluble fraction.

B

The compounds found in the acid soluble pool have a common feature, i.e., molecular weight ranging from 18 to 800 daltons.

C

Acid insoluble pool represents cytoplasmic composition only.

D

Acid soluble pool is due to macromolecules from cytoplasm and cell organelles.

Text Solution

AI Generated Solution

The correct Answer is:
To find the correct statement regarding the acid-soluble and acid-insoluble fractions from a biological sample, we can analyze the information provided in the video transcript step by step. ### Step-by-Step Solution: 1. **Understanding the Process**: - A biological sample (like a leaf or liver) is mixed with trichloroacetic acid and ground up. This mixture is then filtered to separate the components. **Hint**: Remember the role of the acid and the grinding process in breaking down the biological sample. 2. **Identifying the Fractions**: - After filtering, we obtain two fractions: the Acid Soluble Fraction and the Acid Insoluble Fraction. The Acid Soluble Fraction contains smaller molecules that can dissolve in the acid. **Hint**: Focus on the size of molecules that can pass through the filter. 3. **Characteristics of the Acid Soluble Fraction**: - The Acid Soluble Fraction does not contain macromolecules (large molecules greater than 1 kDa). Instead, it contains smaller compounds with molecular weights ranging from 18 to 800 daltons. **Hint**: Recall the definition of macromolecules and their size in daltons. 4. **Evaluating the Statements**: - **Statement 1**: "In Acid Soluble Fraction, bio-macromolecules are not found." This is true because macromolecules are too large to pass through the filter. - **Statement 2**: "The compounds found in the acid-soluble pool have a common feature that is molecular weight ranging from 18 to 80 daltons." This is partially true; the range is actually from 18 to 800 daltons. - **Statement 3**: "Acid insoluble pool represents cytoplasmic composition only." This is incorrect because the acid-insoluble pool can contain various components, not just those from the cytoplasm. - **Statement 4**: "Acid-soluble pool is due to macromolecules from cytoplasm and cell organelle." This is also incorrect, as the acid-soluble pool does not contain macromolecules. 5. **Conclusion**: - The correct statement is that the compounds found in the acid-soluble pool have a common feature of molecular weights ranging from 18 to 800 daltons. Therefore, the correct answer is Statement 2. ### Final Answer: The correct statement is: "The compounds found in the acid-soluble pool have a common feature that is molecular weight ranging from 18 to 800 daltons."
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