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The sticky ends generated by the action ...

The sticky ends generated by the action of Eco RI on insert DNA facilitate the action of which enzyme ?

A

DNA polymerase

B

Taq polymerase

C

Alkaline phosphatase

D

DNA Iigase

Text Solution

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The correct Answer is:
C
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Type of sticky end produced by the action of the EcoRI is

The stickiness of DNA ends faciliates the action of which enzyme

The 'sticky ends' on each of the two strands of a DNA generated by treatement with restriction enzyme facilitate the action of enzyme :

The mode of action of an enzyme is:

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : Stickness of the ends facilitates the action of the enzyme DNA polymerase

Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA polymerase

For the enzyme action

Assertion: The stickiness of ends produced by restriction endonuclease faciliates the action of DNA polymerase Reason :It helps in joining the two DNA strands

PHYSICS WALLAH-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES -NEET PAST 10 YEARS QUESTIONS
  1. The sticky ends generated by the action of Eco RI on insert DNA facili...

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  2. Match the organism with its use in biotechnology

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  3. In gel electrophoresis, separated DNA fragments can be visualized with...

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  4. The sequence that controls the copy number of the linked DNA in the v...

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  5. Which of the following palindromic sequence is recognized by Eco RI ?

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  6. Identify the wrong statement with regard to Restriction Enzymes.

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  7. First discovered restriction endonuclease that always cuts DNA molecul...

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  8. In a mixture, DNA fragments are separated by:

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  9. In recombinant DNA technology antibiotics are used:

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  10. Match the following techniques or instruments with their usage: Se...

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  11. Spooling is:-

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  12. Select the correct statement for continous culture system

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  13. Which one of the following equipmcnts is essentially required for grow...

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  14. DNA precipitation out of a mixture of biomolecules can be achieved by ...

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  15. Following statements describe the characteristics of the enzyme Restri...

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  16. The correct order of steps in Polymerase Chain Reaction (PCR) is

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  17. What is the criterion for DNA fragments movement on agarose gel during...

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  18. The process of separation and purification of expressed protein before...

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  19. A gene whose expression helps to identify transformed cell is known as

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  20. The DNA fragments separated on an agarose gel can be visualised after ...

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  21. Restriction endonucleases are:

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