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A restriction endonuclease breaks down b...

A restriction endonuclease breaks down between
a) Base pairs of a DNA molecule
b) Base pairs of a DNA- RNA
c) Sugar and phosphate components of a nucleic acid molecule
d) Exons & introns of a DNA molecule

A

Base pairs of a DNA molecule

B

Base pairs of a DNA- RNA

C

Sugar and phosphate components of a nucleic acid molecule

D

Exons & introns of a DNA molecule

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The correct Answer is:
To solve the question regarding where a restriction endonuclease breaks down, we can follow these steps: ### Step-by-Step Solution: 1. **Understand the Function of Restriction Endonucleases**: - Restriction endonucleases are enzymes that cut DNA at specific sequences. They are often referred to as "molecular scissors" because they create incisions in the DNA molecule. 2. **Identify the Target of Restriction Endonucleases**: - These enzymes recognize specific sequences in the DNA, known as restriction sites, which typically contain palindromic sequences. 3. **Determine the Location of the Cut**: - The cuts made by restriction endonucleases occur at the sugar-phosphate backbone of the DNA. This means they cleave the bonds between the sugar and phosphate components of the nucleic acid molecule, rather than between the base pairs or within the bases themselves. 4. **Evaluate the Options**: - a) Base pairs of a DNA molecule: This option is incorrect because the enzyme does not cut between the base pairs. - b) Base pairs of a DNA-RNA: This option is also incorrect for the same reason as option a. - c) Sugar and phosphate components of a nucleic acid molecule: This option is correct as it accurately describes where the restriction endonuclease acts. - d) Exons & introns of a DNA molecule: This option is incorrect because restriction endonucleases do not specifically target exons and introns. 5. **Conclusion**: - The correct answer is **c) Sugar and phosphate components of a nucleic acid molecule**.
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PHYSICS WALLAH-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES -NEET PAST 10 YEARS QUESTIONS
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  2. Match the organism with its use in biotechnology

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  3. In gel electrophoresis, separated DNA fragments can be visualized with...

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  4. The sequence that controls the copy number of the linked DNA in the v...

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  5. Which of the following palindromic sequence is recognized by Eco RI ?

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  6. Identify the wrong statement with regard to Restriction Enzymes.

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  7. First discovered restriction endonuclease that always cuts DNA molecul...

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  8. In a mixture, DNA fragments are separated by:

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  9. In recombinant DNA technology antibiotics are used:

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  10. Match the following techniques or instruments with their usage: Se...

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  11. Spooling is:-

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  12. Select the correct statement for continous culture system

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  13. Which one of the following equipmcnts is essentially required for grow...

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  14. DNA precipitation out of a mixture of biomolecules can be achieved by ...

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  15. Following statements describe the characteristics of the enzyme Restri...

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  16. The correct order of steps in Polymerase Chain Reaction (PCR) is

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  17. What is the criterion for DNA fragments movement on agarose gel during...

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  18. The process of separation and purification of expressed protein before...

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  19. A gene whose expression helps to identify transformed cell is known as

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  20. The DNA fragments separated on an agarose gel can be visualised after ...

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  21. Restriction endonucleases are:

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