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In presence of chromogenic substrate rec...

In presence of chromogenic substrate recombinant bacteria gives

A

Red coloured colonies

B

Colourless colonies

C

Blue colonies

D

Green colonies

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The correct Answer is:
B
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" In E-coli,if the gene for "beta" galactosidase enzyme is inactivated by insertion of DNA,then in presence of chromogenic substract recombinant will give "

In bacterial culture some of the colonies produced blue colour in the presence of chromogenic substrate and some did not due to presence or absence of an inert (rDNA) in the coding sequence of B galactosidase. (a) Mention the mechanism and steps involved in above experiment. (b) How is it advantageous over simultaneous plating on two plates having different antibiotics.

During insetional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the emzyme

The presence of a chromogenic substrate gives blue coloured colonies of bacteria. Insertional inactivation would stop product of which of the following enzyme such that colonies do not produce any colour.

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as (i) Recombinants if colonies are blue-coloured (ii) Recombinants if colonies are blue coloured (iii) Non-recombinants if colonies are blue-coloured (iv) Non - recombinants if colonies are white coloured

If desired gene is inserted within the coding sequence of an enzyme, β-galactosidase in a vector then colonies of bacteria which do not produce any colour in the presence of a chromagenic substrates are :-

Assertion (A) In presence of enzyme, substrate molecule can be attacked by the reagent effectively. Reason (R) Active sites of enzymes hold the substrate molecule in a suitable position.

PHYSICS WALLAH-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES -LEVEL-2
  1. Function of restriction endonuclease enzyme is

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  2. Which of the followoing restriction endonuclease enzyme produce blunt ...

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  3. A bacterium modifies its DNA by adding methyl groups to the DNA, It do...

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  4. Select the incorrect statement for continous culture system

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  5. Match the columns and find out the correct combinations:

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  6. In which type of bioreactor air bubbles dramatically increases the oxy...

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  7. Which of following feature is not necessary for cloning vector

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  8. Which of the following step is not involved in basic steps in genetica...

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  9. Which of the following step is not involved in basic steps in genetica...

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  10. In r-DNA technology or genetic engineering elution means-

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  11. Read the following statements for restriction enzymes and choose optio...

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  12. Which is correctly matched?

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  13. In presence of chromogenic substrate recombinant bacteria gives

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  14. An ideal cloning vector should have. A. Low molecular weight B. Or...

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  15. Restriction endonucleases are used in genetic engineering to form

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  16. Which of the following tools of recombinant DNA technology is incorrec...

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  17. If E.coli is grown in a medium with a recombinant DNA with ampicillin ...

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  18. Which of the following statement is incorrect?

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  19. Which technique is used to check the progression of restriction enzyme...

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  20. Which enzyme is not related correctly with its function?

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