Home
Class 12
BIOLOGY
An ideal cloning vector should have. A...

An ideal cloning vector should have.
A. Low molecular weight
B. Origin of replication
C. Desired genes
D. Resistance to antibiotics

A

C and D

B

A,B and D

C

A and C

D

B and C

Text Solution

AI Generated Solution

The correct Answer is:
To determine the characteristics of an ideal cloning vector, we need to analyze each option provided in the question: 1. **Low Molecular Weight (Option A)**: - An ideal cloning vector should have a low molecular weight. This is important because a lighter vector is easier to manipulate and can be replicated more efficiently within the host organism. Therefore, this characteristic is beneficial for cloning. 2. **Origin of Replication (Option B)**: - The origin of replication (often denoted as OREC) is a crucial feature of any cloning vector. It is the specific sequence where replication begins, allowing the vector to be copied within the host cell. This is essential for the propagation of the vector and the genes it carries. 3. **Desired Genes (Option C)**: - While a cloning vector may carry desired genes, this is not a defining characteristic of an ideal cloning vector itself. The primary function of a cloning vector is to facilitate the cloning process, and the presence of desired genes is more related to the purpose of cloning rather than the vector's ideal properties. 4. **Resistance to Antibiotics (Option D)**: - Antibiotic resistance is a significant feature of an ideal cloning vector. It allows for the selection of successfully transformed cells. For example, if a vector carries a gene for resistance to ampicillin, only those cells that have taken up the vector will survive in an environment containing ampicillin. This is crucial for identifying which cells have successfully incorporated the vector. Based on the analysis of the options: - The ideal cloning vector should have: - **Low Molecular Weight (A)**: Yes - **Origin of Replication (B)**: Yes - **Desired Genes (C)**: No - **Resistance to Antibiotics (D)**: Yes Thus, the correct answer is that an ideal cloning vector should have options A, B, and D. **Final Answer**: A, B, and D are characteristics of an ideal cloning vector. ---
Promotional Banner

Topper's Solved these Questions

  • BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

    PHYSICS WALLAH|Exercise NEET PAST 10 YEARS QUESTIONS |47 Videos

  • BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

    PHYSICS WALLAH|Exercise NEET PAST 10 YEARS QUESTIONS |47 Videos

  • BIOTECHNOLOGY AND ITS APPLICATIONS

    PHYSICS WALLAH|Exercise NEET Past 10 Years Questions|31 Videos

  • BODY FLUIDS AND CIRCULATION

    PHYSICS WALLAH|Exercise NEET Past 10 Years Questions|28 Videos

Similar Questions

Explore conceptually related problems

Which of the following statement // s regarding nucleic acids is // are wrong ? Amplification of gene of interest is done by polymerase Chain Reaction. Origin of replication (ori) is a sequence from where RNA replication starts. The tumour inducing (Ti) plasmid of Agrobacterium tumifaciens can be modified into a cloning vector . DNA technology involves the use of restriction endonucleases. Biolistics or gene gun is suitable to introduce DNA to plant cells.

Which of the following features are required to facilitate cloning into a vector ? a. Origin of replication (ori) b. Selectable marker c. Cloning site

Draw a schematic diagram of the E. coli cloning vector Pbr322 and mark the following in it : (a) ori (b) rop (c) ampicillin resistance gene (d) tetracycline resistance gene (e) restriction site BamHI (f) restriction site EcoRI

Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites, Ori, antibiotic resistance genes & ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant. Identify the missing steps (A,B and C) with regard ot following statements and select the correct option. (i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. (ii) Selection of transformed cells. (iii) Transferring the recombinant DNA molecules to teh target cells.

Which one is correct answer for plasmid (i) It can be readily isolated from cells (ii) It possesses a single recognition site for one restriction enzyme (iii) It should have high molecular weight (iv) Plasmids and bacteriophages have the ability to replicate within bacteria

Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out fron agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

Assertion : All expression vectors are cloning vectors and vica versa. Reason : Expression vectors have at least the regulatory sequences i.e., promotes, operators, ribosomal binding sites, etc having optimum function in the chosen control but not origin of replication.

PHYSICS WALLAH-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES -LEVEL-2
  1. Function of restriction endonuclease enzyme is

    Text Solution

    |

  2. Which of the followoing restriction endonuclease enzyme produce blunt ...

    Text Solution

    |

  3. A bacterium modifies its DNA by adding methyl groups to the DNA, It do...

    Text Solution

    |

  4. Select the incorrect statement for continous culture system

    Text Solution

    |

  5. Match the columns and find out the correct combinations:

    Text Solution

    |

  6. In which type of bioreactor air bubbles dramatically increases the oxy...

    Text Solution

    |

  7. Which of following feature is not necessary for cloning vector

    Text Solution

    |

  8. Which of the following step is not involved in basic steps in genetica...

    Text Solution

    |

  9. Which of the following step is not involved in basic steps in genetica...

    Text Solution

    |

  10. In r-DNA technology or genetic engineering elution means-

    Text Solution

    |

  11. Read the following statements for restriction enzymes and choose optio...

    Text Solution

    |

  12. Which is correctly matched?

    Text Solution

    |

  13. In presence of chromogenic substrate recombinant bacteria gives

    Text Solution

    |

  14. An ideal cloning vector should have. A. Low molecular weight B. Or...

    Text Solution

    |

  15. Restriction endonucleases are used in genetic engineering to form

    Text Solution

    |

  16. Which of the following tools of recombinant DNA technology is incorrec...

    Text Solution

    |

  17. If E.coli is grown in a medium with a recombinant DNA with ampicillin ...

    Text Solution

    |

  18. Which of the following statement is incorrect?

    Text Solution

    |

  19. Which technique is used to check the progression of restriction enzyme...

    Text Solution

    |

  20. Which enzyme is not related correctly with its function?

    Text Solution

    |