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First discovered restriction endonucleas...

First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is

A

Adenososine deaminase

B

Thermostable DNA polymerase

C

Hind II

D

EcoRI

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The correct Answer is:
C
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Explore conceptually related problems

Restriction endonuclease Hind II always cuts DNA molecule at a particular point by recognising a specific sequence of

EcoRI always cut DNA molecules at a particular point by recognizing a specific sequence between :

Assertion : The first restriction endonuclease was Hine II Reason :Hind II always cut DNA molecules at a particular point by recongnising a specific sequence of six base pairs.

Which of the following statements are correct ? (i) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site, but between the same two bases on the opposite strands. (ii) Hind ll always cuts DNA molecules at a particular point by reconginisng a specific sequence of six base pairs. (iii) Separated DNA fragments cannot be visualised without staining on an agarose gel electrophoresis. (iv) 'Ori' is the sequence responsible for controlling the copy number. (v) DNA is a positively charged molecule.

The first discovered restriction endonuclease enzyme was :-

Hind II always join DNA molecules at specific site by recognising a particular sequence of six base pairs.

Hind-II always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs. This specific base sequence is known as the................ .

How many statements are correct? (a) Hind II always cut DNA molecules at particular point by recognizing a specific sequence of 6 bases. (b)After the restriction endonuclease action, single stranded portions which are overhanging stretches called sticky ends because they form nucleotide bonds with their complementary cut counter part. (c)The separated bands of DNA are cut out from the agarosegel and extracted from gel piece. This step is known as spooling. (d)Taq polymerase is used between annealing and extension of r-DNA technology.

EcoRI always cuts DNA molecules at a particluar point by recognizing a specific restriction sequence, the sticky ends fromed after digestion have the sequence

PHYSICS WALLAH-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES -NEET PAST 10 YEARS QUESTIONS
  1. Which of the following palindromic sequence is recognized by Eco RI ?

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  2. Identify the wrong statement with regard to Restriction Enzymes.

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  3. First discovered restriction endonuclease that always cuts DNA molecul...

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  4. In a mixture, DNA fragments are separated by:

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  5. In recombinant DNA technology antibiotics are used:

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  6. Match the following techniques or instruments with their usage: Se...

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  7. Spooling is:-

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  8. Select the correct statement for continous culture system

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  9. Which one of the following equipmcnts is essentially required for grow...

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  10. DNA precipitation out of a mixture of biomolecules can be achieved by ...

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  11. Following statements describe the characteristics of the enzyme Restri...

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  12. The correct order of steps in Polymerase Chain Reaction (PCR) is

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  13. What is the criterion for DNA fragments movement on agarose gel during...

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  14. The process of separation and purification of expressed protein before...

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  15. A gene whose expression helps to identify transformed cell is known as

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  16. The DNA fragments separated on an agarose gel can be visualised after ...

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  17. Restriction endonucleases are:

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  18. Which of the following restriction enzymes produces blunt ends ?

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  19. Which of the following is not a component of downstream processing

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  20. A foreign DNA and plasmid cut by the same restriction endonuclease can...

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