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Regarding transgenic tobacco plants whic...

Regarding transgenic tobacco plants which is resistance to the nematode meloidegyne incognita, the correct statements are
A. The nematode parasite can't infect the transgenic host
B. The nematode parasite can't produce mRNA in host cells
C. The host cells contain the vector Agrobacterium
D. Host cells produce both sense and antisense RNA of a particular gene of the nematode

A

A and C only

B

B and D only

C

D only

D

All of these

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AI Generated Solution

The correct Answer is:
To determine the correct statements regarding transgenic tobacco plants resistant to the nematode Meloidogyne incognita, let's analyze each option step by step: ### Step 1: Understanding Transgenic Tobacco Plants Transgenic tobacco plants have been genetically modified to express certain traits, in this case, resistance to the nematode Meloidogyne incognita. This is typically achieved by inserting a gene of interest into the plant's genome using a vector, often derived from the bacterium Agrobacterium tumefaciens. ### Step 2: Analyzing Each Statement - **Statement A:** "The nematode parasite can't infect the transgenic host." - **Analysis:** This statement is true. The purpose of creating a transgenic plant is to provide resistance against the nematode, meaning that the nematode should not be able to infect the plant. - **Statement B:** "The nematode parasite can't produce mRNA in host cells." - **Analysis:** This statement is also true. The transgenic plant produces both sense and antisense RNA that can interfere with the nematode's mRNA synthesis, preventing it from producing necessary proteins for its survival and reproduction. - **Statement C:** "The host cells contain the vector Agrobacterium." - **Analysis:** This statement is misleading. While Agrobacterium is used to introduce the gene into the plant cells, it is not present in the host cells after the transformation process. Therefore, this statement is false. - **Statement D:** "Host cells produce both sense and antisense RNA of a particular gene of the nematode." - **Analysis:** This statement is true. The transgenic tobacco plants are designed to produce both sense and antisense RNA, which can lead to RNA interference (RNAi) against the nematode's mRNA, effectively blocking its function. ### Conclusion Based on the analysis: - **Correct Statements:** A, B, and D are true. - **Incorrect Statement:** C is false. ### Final Answer The correct statements regarding transgenic tobacco plants resistant to Meloidogyne incognita are: - A. The nematode parasite can't infect the transgenic host. - B. The nematode parasite can't produce mRNA in host cells. - D. Host cells produce both sense and antisense RNA of a particular gene of the nematode.
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Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells):

Assertion: The nematode parasite could not survive in a transgenic plant expressing specific interfering RNA Reason :The sense & anti -sense RNA in host are complemenatry & forms a ds RNA, thus silenced the specific m RNA of nematod

Explain the events that occur in the host cell on introduction of nematode-resistant gene into the tabacco plant by using Agrobacterium vectors

Using agrobacterium vectors, nematod specific genes were introduced into host plant and this DNA produces :-

RNA interference (RNAi) technique has been devised to protect the plants from the nematode. In this technique , mRNA, of nematode, is silenced by _______ produced by the host plant :

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