In a maternity clinic, for some reasons the authorities are not able to hand over the two new-borns to their respective real parents. Name and describe the technique that you would suggest to sort out the matter.

Explanation

To sort out the matter DNA finger printing of parents and new born are to be conducted to hand over the newborns to their real biological parents. DNA fingerprints can be carried out by taking DNA from extremely minute amounts of blood, semen, hair bulb or any other cells of the body. The major steps are as follows:
(i) isolation of DNA from nuclei of cells
(ii)If the content is limited, DNA can be amplified using Polymerase Chain Reaction
(iii)Digestion of DNA by restriction endonucleases that cuts them into fragments electrophoresis.
(iv)Separation of DNA fragments according to their size by gel
(v) The separated fragments of DNA in the gel are copied on to a synthetic membranes such as nylon or nitrocellulose by southern blotting technique
(vi) Special DNA-probes are made in the laboratory and are made radioactive by labelling with radioactive isotopes. The radioactive DNA probes bind to the repetitive sequences on the nylon membrane
(vii) Detection of DNA fragments by autoradiography An X-ray film is exposed to the nylon membrane to mark the places where the radioactive DNA-probes have bound to the DNA fragments. These places are marked as dark bands when X-ray film ts developed. These dark bands on X-ray film represent the DNA fingerprints.
Bands of new born will have resemblance with their respective parents