Proteins that hep to open up DNA helix i...

Proteins that hep to open up DNA helix in front of the replication fork in prokaryotes are

topoisomerases

unwindases (helicases)

polymerase 1

exonucleases and endocuclease

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A 3' ______________ 5' B C 5' _______________ 3'D AB and CD represent two strands of a DNA molecule. When this molecule undergoes replication, forming a replication fork between A and C in the above. (i) Name the template strands for replication. (ii) Using which strand as the template, will there be continuous synthesis of a complementary DNA strand? (iii) Complementary to which strand will Okazaki segments get synthesised and discontinuous synthesis will occur (iv) What are template strands and Okazaki pieces? (v) In which direction is a new strand synthesized?

Draw a labelled schematic sketch of replication fork of DNA. Explain the role of the enzymes inolved in DNA replication.

Eukaryotes differ from prokaryotes in the mechanism of DNA replication due to

Consider the following events in DNA replication 1. Formation of RNA primers by RNA polymerase. 2. Removal of RNA primers and formation of Okazaki fragments. Formation of DNA strands on RNA primers by DNA poly III complex. 4. Recognition of initiation point by initiator protein. 5. Dissociation of hydrogen bonds and opening of a 'bubbles' in the duplex by unwinding proteins. 6. Filling up of the gaps between Okazaki fragments and formation of DNA strand. The correct sequence of these events is:-

Go through the following statements: (i) a typical nucleosome contains 400 bp of DNA helix. (ii) The positively charged DNA is wrapped around the negatively charged histoned octamer to form a structure called nucleosome. (iii) Histones are rich in the basic amino acid residues asparagine ad valine (iv) The packaging of chromatin at higher level requires additional set of proteins that collectively are referred to as nonhistone chromosomal proteins. Find out the wrong statements:-

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