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Assertion : E.coli having pBR322 with DN...

Assertion : E.coli having pBR322 with DNA insert at BamH 1 site cannot grow in medium containing tetracycline.
Reson: Recognition site for BamH 1 is present in tetR region of pBR322.

A

Both assertion and reason are true, and reason is the correct explanation of assertion.

B

Both assertion and reason are true, but reason is the correct explanation of assertion

C

Assertion is true but reason is false.

D

Both asertion and reason are false.

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Two questions consist of two statements each, printed as Assertion and Reason. While answering these questions, you required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is a correct explanation of Association. If both Association and Reason are true and Reasons is not a correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertions and Reasongs are false. Assertion: Recognition site should be preferably single and responsive to commonly used restriction enzyme. Reason:In pBR 322 alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.

A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?

MBD-MODEL TEST PAPER -1 -EXERCISE
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  12. How is a continous culture system maintained in bioreactors and why?

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  14. What is EcoRI? How does EcoRI differ from an exonuclease?

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  15. What are two core techniques that enabled birth of biotechnology?

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