Draw the vector DNA and a foreign DNA showing the sites where EcoRI has acted to form the sticky ends.

A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.

Name the enzyme used to link the sticky ends of DNA fragments.

Name the enzyme used to link the sticky ends of DNA fragments.

A hollow charged conductor has a tiny hole cut into its surface. Show that the electric field in the hole is (sigma//2epsilon_0) hatn , where hatn is the unit vector in the outward normal direction, and sigma is the surface charge density near the hole.

How are 'Sticky ends' formed on a DNA strand? Why are these so called?

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

Show that the normal component of electrostatic field has a discontinuity from one side of a charged surface to another given by (E_2 - E_1) hatn= (sigma)/(epsilon_0) where hatn is a unit vector normal to the surface at a point and is the surface charge density at that point. (The direction of hat n is from side 1 to side 2.) Hence show that just outside a conductor, the electric field is sigma hatn // epsilon_0

Consider an over all neutral sphere of radius R. This sphere has a point charge +Q at its centre and this positive charge is surrounded by a uniform density rho of negative charge upto a radius R. Use Gauss' law to obtain expressions for the electric field of this at a point distant r form its centre. where r < R. show that these two expressions give identical results for the elctric field at r = R.

Consider an over all neutral sphere of radius R. This sphere has a point charge +Q at its centre and this positive charge is surrounded by a uniform density rho of negative charge upto a radius R. Use Gauss' law to obtain expressions for the electric field of this at a point distant r form tis centre. where r > R. show that these two expressions give identical results for the elctric field at r = R

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using: