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EcoRI is a restriction endonuclease. How...

EcoRI is a restriction endonuclease. How is it named so?

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Restriction endonucleases:

How are restriction endonuclease enzymes named ?

Restriction endonucleases are useful in

How are restriction endonuclease enzymers are named? Write Example.

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:

A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.

Restriction endonucleases are so called because they:

What are endonucleases?

Restriction enzymes:

MBD-BIOTECHNOLOGY AND ITS APPLICATIONS-EXERCISE
  1. What are Cry proteins? Name an organism that produce it. How has man e...

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  2. What do you understand by the term selectable marker?

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  3. EcoRI is a restriction endonuclease. How is it named so?

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  4. Write the sequence of DNA bases that this enzyme recognises?

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  5. Explain with reference PCR A specific enzyme helps in amplification...

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  6. Explain with reference PCR Explain its role in molecular diagnosis.

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  7. With a simple sketch show the steps of recombinant DNA technology.

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  8. With an example, Explain how biotechnology has been applied in each of...

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  9. Which example, explain how biotechnology has been applied in each of t...

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  10. How DNA fragments of different sizes can be separated and isolated?

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  11. Use of GM plants is not universally accepted . Write down some of its ...

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  12. How does a transgenic plant of tobaaco protect it self from nematode ...

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  13. EcoRI enzymes form 'stickly ends' in cut DNA strands Explain .

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  14. A bacterium ashowed mutation and the mutant gene was found to be benef...

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  15. Nam two genetically modified crops.

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  16. what does the following figure depict?

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  17. What is meant by largest and smallest figure in gel electrophoresis?

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  18. Name the compound used to visualise them DNA fragments in gel electoph...

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  19. Define elution.

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  20. Transgenic animals are proving to be a boon in various areas. Justify.

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