EcoRI enzymes form 'stickly ends' in cut DNA strands Explain .

Enzyme that cuts DNA is

Name the enzyme used to link the sticky ends of DNA fragments.

Name the enzyme used to link the sticky ends of DNA fragments.

How are 'Sticky ends' formed on a DNA strand? Why are these so called?

Name the enzyme that joins the short pieces in the lagging strand during synthesis of DNA.

A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.

The restriction enzymes that are used in construction of recombinant Dna molecule are endonucleases which cut the DNA at 'specific recognition sequence'. What would be the disadvantage if they would not cut the DNA at specific -recognition sequence?