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Assertion .Gram negative bacteria do not...

Assertion .Gram negative bacteria do not retain the when washed with alcohol.
Reason . The outer face of outer membrane of Gram negative bacteria contain lipopolysaccharides a part of which is integrated with membrane lipids.

A

If both Assertion & Reason are true and the reason is the correct explanation of the assertion.

B

It both Assertion & Reason are true but the reason is not the correct explanation of the assertion.

C

If Assertion is true statement but Reason is false.

D

It both Assertion and Reason are false statements.

Text Solution

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The correct Answer is:
To solve the question regarding the assertion and reason related to Gram-negative bacteria, we will break it down step by step. ### Step-by-Step Solution 1. **Understanding the Assertion**: - The assertion states: "Gram-negative bacteria do not retain the stain when washed with alcohol." - This statement is true. Gram-negative bacteria have a thinner peptidoglycan layer and an outer membrane that can be disrupted by alcohol, leading to the loss of the crystal violet stain used in Gram staining. 2. **Understanding the Reason**: - The reason states: "The outer face of the outer membrane of Gram-negative bacteria contains lipopolysaccharides, a part of which is integrated with membrane lipids." - This statement is also true. Gram-negative bacteria have an outer membrane rich in lipopolysaccharides (LPS), which contributes to their structural integrity and is a crucial factor in their staining characteristics. 3. **Connecting Assertion and Reason**: - Now, we need to determine if the reason provided explains the assertion. - The presence of lipopolysaccharides in the outer membrane of Gram-negative bacteria is indeed a reason why they do not retain the stain when washed with alcohol. The alcohol disrupts the outer membrane, allowing the stain to wash away. 4. **Conclusion**: - Since both the assertion and reason are true, and the reason correctly explains the assertion, we conclude that the correct answer is: - **Option 1**: Both assertion and reason are true, and the reason is the correct explanation of the assertion.
To solve the question regarding the assertion and reason related to Gram-negative bacteria, we will break it down step by step. ### Step-by-Step Solution 1. **Understanding the Assertion**: - The assertion states: "Gram-negative bacteria do not retain the stain when washed with alcohol." - This statement is true. Gram-negative bacteria have a thinner peptidoglycan layer and an outer membrane that can be disrupted by alcohol, leading to the loss of the crystal violet stain used in Gram staining. ...
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