Recombinant DNA technology involves several sleps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the break down of fungal cell, plant cell and bacterial cell respectively?

Options

A. lysozyme, lipases, trypsin
B. chitinase, cellulase, lysozyme
C. chitinase, cellulase, trypsin
D. trypsin, lipases, cellulase

Explanation

To solve the question regarding the enzymes used for the breakdown of fungal, plant, and bacterial cells in the context of recombinant DNA technology, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Structure of Cell Walls**:
- **Fungal Cell Wall**: The fungal cell wall is primarily composed of chitin, a polysaccharide.
- **Plant Cell Wall**: The plant cell wall is mainly made up of cellulose, another type of polysaccharide.
- **Bacterial Cell Wall**: The bacterial cell wall consists of a peptidoglycan layer.

2. **Identifying the Enzymes**:
- **For Fungal Cells**: To break down chitin in the fungal cell wall, the enzyme **chitinase** is used. Chitinase specifically hydrolyzes the chitin polymer.
- **For Plant Cells**: To break down cellulose in the plant cell wall, the enzyme **cellulase** is required. Cellulase acts on cellulose to facilitate its breakdown.
- **For Bacterial Cells**: To break down the peptidoglycan layer in bacterial cell walls, the enzyme **lysozyme** is utilized. Lysozyme cleaves the bonds in the peptidoglycan, leading to cell wall lysis.

3. **Conclusion**:
- The enzymes used for the breakdown of the respective cell walls are:
- Fungal cell: **Chitinase**
- Plant cell: **Cellulase**
- Bacterial cell: **Lysozyme**

### Final Answer:
- The enzymes used for the breakdown of fungal, plant, and bacterial cells respectively are **Chitinase, Cellulase, and Lysozyme**.