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Assetion: Insertion of recombinant DNA w...

Assetion: Insertion of recombinant DNA within the coding sequence of `beta-`galactosidase result n colourless colonies.
Reason : Presence of insert results in inactivation of enzyme `beta-`galactosidase known as insertional inactivation

A

Assertion is correct, reason is correct, reason is a correct explanation for assertion.

B

Assertion is correct, reason is correct, reason is not a correct explanation for assertion

C

Assertion is correct, reason is incorrect

D

Assertion is incorrect, reason is correct.

Text Solution

Verified by Experts

The correct Answer is:
A
Alternative markers have been developed that can differentiate recombinants from chromogenicsubstrate.Theplasmidinthe bacteria, lacking an insert produces blue coloured colonies,whilethoseplasmidswith an insert donot produce any colour due to insertional inactivation of the enzyme, `beta` galactosidase.
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