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Draw agarose gel electrophoresis apparat...

Draw agarose gel electrophoresis apparatus. Description is not required.

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Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

A mixture containing DNA fragments A,B,C and D, with molecular weights of A + B = C,A gt B and D gt C , was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anode sides of the gel would be (a) D,C,A,B (b) A,B,C,D (c) C,B,A,D (d) B,A,D,C

Gel electrophoresis separates the DNA on the basis of its ---.

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A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease, When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while, linear DNA shows two fragments. Explain.

Which of the following statements are correct ? (i) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site, but between the same two bases on the opposite strands. (ii) Hind ll always cuts DNA molecules at a particular point by reconginisng a specific sequence of six base pairs. (iii) Separated DNA fragments cannot be visualised without staining on an agarose gel electrophoresis. (iv) 'Ori' is the sequence responsible for controlling the copy number. (v) DNA is a positively charged molecule.