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A plasmid DNA and a linear DNA (both of ...

A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease, When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while, linear DNA shows two fragments. Explain.

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This is because plasmid is a circular DNA molecule. When cut with enzyme, it becomes linear, but does not get fragmented. Whereas, a linear DNA molecule gets cut into two fragments. Hence, a single DNA band is observed for plasmid while, two DNA bands are observed for linear DNA in agarose gel.
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