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How are recombinant vectors created?...

How are recombinant vectors created?

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The vector DNA is cut at a particular restriction site using a restriction enzyme (to cut the desired DNA segment). The alien DNA is then linked with the plasmid DNA using an enzyme called ligase to form the recombinant vector.
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ARIHANT PUBLICATION-PRINCIPLES AND PROCESSES OF BIOTECHNOLOGY -Part II (Questions For Practice) (Short Answer Type Questions)
  1. Write the role of 'ori' and 'restriction site' in a cloning vector PBR...

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  2. Write a short note on plasmids.

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  3. You have chosen a plasmid as vector for cloning your gene. However, th...

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  4. Draw a schematic sketch of PBR322 plasmid and label the following in i...

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  5. Draw a schematic sketch of PBR322 plasmid and label the following in i...

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  6. Draw a schematic sketch of PBR322 plasmid and label the following in i...

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  7. How are recombinant vectors created?

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  8. For creating one recombinant vector only one type of restriction endon...

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  9. How bacterial cells are made competent to take up DNA?

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  10. Describe the role of CaCl, in the prepration of competent cell.

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  11. PCR is a useful tool for early diagnosis of an infectious disease. Com...

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  12. Explain, how to find whether an Ecoli bacterium has transformed or not...

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  13. What does the ampicillin-resistant gene act as ?

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  14. Name the source of the DNA polymerase used in PCR technique. Mention, ...

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  15. Give the name of the organism from where the thermostable DNA polymera...

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  16. Rearrange the following in the correct sequence to accomplish an impor...

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  17. While doing a PCR, denaturation step is missed. What will be its effec...

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  18. A schematic representation of PCR up to the extension stage is given b...

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  19. A schematic representation of PCR up to the extension stage is given b...

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  20. A schematic representation of PCR up to the extension stage is given b...

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