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Explain, how to find whether an Ecoli ba...

Explain, how to find whether an Ecoli bacterium has transformed or not, when a recombinant DNA bearing ampicillin-resistance gene is transferred into it.

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The recombinant/transformant can be selected out from the non-recombinants/ non-transformants by plating the transformants on ampicillin-containing medium. The transformants will grow in it, while the non-transformants will not grow.
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If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grown and the untransformed recipient cells will die. The ampicillin resistant gene in this case is called as 1) selectable marker 2) recombinant protein 3) cloning site 4) chemical scalpels

Explain two situations, when independent assortment of genes occurs, resulting is 50% recombinants

Read the given statements and select the correct option. Statement 1: Both bacteria and yeast multiply very fast to form huge populations which express the desired gene. Statement 2: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryotes).

Read the following statements and select the incorrect ones. (i) When the transformed cells on agar plates containing ampicillin are spread, both transformed and untransformed cells will grow. (ii) Restriction enzymes are used in isolation and separation of DNA from other macromolecules. (iii) Downstream processing is one of the steps of rDNA technology. (iv) Disarmed pathogen vectors are also used in transfer of rDNA into the host. a) (i) and (iii) b) (iii) and (iv) c) (i) and (iii) d) (i) and (ii)

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reason could be (a) Human gene may have intron which bacteria cannot process (b) Amino acid codons for humans and bacteria are different (c) Human protein is formed but degraded by bacteria (d) all of the above

How recombinant DNA technology is useful is detecting the presence of mutated genes?

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant. Identify the missing steps (A,B and C) with regard ot following statements and select the correct option. (i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. (ii) Selection of transformed cells. (iii) Transferring the recombinant DNA molecules to teh target cells.

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Explain how a galvanometer is converted into an ammeter. Find the expression for low resistance.

ARIHANT PUBLICATION-PRINCIPLES AND PROCESSES OF BIOTECHNOLOGY -Part II (Questions For Practice) (Short Answer Type Questions)
  1. Write the role of 'ori' and 'restriction site' in a cloning vector PBR...

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  2. Write a short note on plasmids.

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  3. You have chosen a plasmid as vector for cloning your gene. However, th...

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  4. Draw a schematic sketch of PBR322 plasmid and label the following in i...

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  5. Draw a schematic sketch of PBR322 plasmid and label the following in i...

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  6. Draw a schematic sketch of PBR322 plasmid and label the following in i...

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  7. How are recombinant vectors created?

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  8. For creating one recombinant vector only one type of restriction endon...

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  9. How bacterial cells are made competent to take up DNA?

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  10. Describe the role of CaCl, in the prepration of competent cell.

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  11. PCR is a useful tool for early diagnosis of an infectious disease. Com...

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  12. Explain, how to find whether an Ecoli bacterium has transformed or not...

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  13. What does the ampicillin-resistant gene act as ?

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  14. Name the source of the DNA polymerase used in PCR technique. Mention, ...

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  15. Give the name of the organism from where the thermostable DNA polymera...

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  16. Rearrange the following in the correct sequence to accomplish an impor...

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  17. While doing a PCR, denaturation step is missed. What will be its effec...

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  18. A schematic representation of PCR up to the extension stage is given b...

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  19. A schematic representation of PCR up to the extension stage is given b...

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  20. A schematic representation of PCR up to the extension stage is given b...

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