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Name the source of the DNA polymerase us...

Name the source of the DNA polymerase used in PCR technique. Mention, why it is used ?

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Bacterium Thermus aquaticus is a source of enzyme Tag polymerase. As, it is a thermostable enzyme and works at high temperature, it is used to amplify DNA in vitro by PCR. The amplified fragment of desired DNA can be used to ligare with the vector for further cloning .
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ARIHANT PUBLICATION-PRINCIPLES AND PROCESSES OF BIOTECHNOLOGY -Part II (Questions For Practice) (Short Answer Type Questions)
  1. Write the role of 'ori' and 'restriction site' in a cloning vector PBR...

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  2. Write a short note on plasmids.

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  3. You have chosen a plasmid as vector for cloning your gene. However, th...

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  4. Draw a schematic sketch of PBR322 plasmid and label the following in i...

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  5. Draw a schematic sketch of PBR322 plasmid and label the following in i...

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  6. Draw a schematic sketch of PBR322 plasmid and label the following in i...

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  7. How are recombinant vectors created?

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  8. For creating one recombinant vector only one type of restriction endon...

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  9. How bacterial cells are made competent to take up DNA?

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  10. Describe the role of CaCl, in the prepration of competent cell.

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  11. PCR is a useful tool for early diagnosis of an infectious disease. Com...

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  12. Explain, how to find whether an Ecoli bacterium has transformed or not...

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  13. What does the ampicillin-resistant gene act as ?

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  14. Name the source of the DNA polymerase used in PCR technique. Mention, ...

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  15. Give the name of the organism from where the thermostable DNA polymera...

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  16. Rearrange the following in the correct sequence to accomplish an impor...

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  17. While doing a PCR, denaturation step is missed. What will be its effec...

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  18. A schematic representation of PCR up to the extension stage is given b...

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  19. A schematic representation of PCR up to the extension stage is given b...

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  20. A schematic representation of PCR up to the extension stage is given b...

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