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Restriction endonuclease...

Restriction endonuclease

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Restriction endonuclease These are specific enzymes, which recognise specific sequences called, recognition sequences on DNA and make double-stranded cuts either within the recognition sequence or at a variable distance from the recognition sequence.
They act as scissors and therefore, often are referred to as molecular scissors. The point of cleavage is known as a restriction site. There will be as many restriction sites as the number of recognition sites. The REs that are used in the recombinant DNA technology fall under class-II. These make double-stranded symmetrical cuts within recognition sequences, generating cohesive or blunt ends.
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Read the given statements and select the correct option, Statement 1 : Restriction endonuclease enzymes recognise a specific palindromic nucleotide sequence in the DNA Statement 2: Restriction endonuclease enzymes are called as molecular scissors or biological scissors.

During insertional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the enzyme 1) α -glucosidase 2) restriction endonuclease 3) β -galactosidase 4) Taq polymerase

Read the following statements and select the correct ones. (i) Same kind of sticky ends are produced when a DNA has been cut by different restriction enzymes. (ii) Exonucleases make cuts at specific positions within the DNA. (iii) Hind ll was the first restriction endonuclease to be isolated. (iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA. (v) Presence of more than one recognition sites for a enzyme within the vector facilitates the gene cloning.

Match the scientists in column I with their related discoveries in column II and select the correct option from the given codes. {:(ColumnI, Column II),(A."Kary Mullis",(i)"Father of genetic engineering"),(B."Paul Berg",(ii)"Nobel prize for the discovery of restriction endonucleases"),(C."Stanley Cohen and Herbert Boyer",(iii)"Developed polymerase chain reaction"),(D."Arber smith and Nathan",(iv)"Isolated an antibiotic resistance gene from a plasmid of the bacterium Salmonella typhimurium"):}

How does a restriction nuclease function ? Explain.

Type-II Restriction enzymes cleave DNA fragments at which site

Restriction enzymes are used to cut single-stranded DNA.

Assertion : Restriction enzymes recongise palindromic sequences. Reason : Palindromic sequences read same in both directions of the two strand.

Which of the following statements does not hold true for restriction enzyme? (a) It recongnises a palindromic nucleotide sequence (b) It is an endonuclease. (c) It is isolated from viruses (d) It produces the same kind of sticky ends in different DNA molecules