Home
Class 12
BIOLOGY
The screening procedure for transformant...

The screening procedure for transformants involving antibiotic resistance properties.

Text Solution

Verified by Experts

Insertional inactivation
Promotional Banner

Topper's Solved these Questions

  • PRINCIPLES AND PROCESSES OF BIOTECHNOLOGY

    ARIHANT PUBLICATION|Exercise Chapter Practice (Short Answer Type-I Questions) |5 Videos

  • PRINCIPLES AND PROCESSES OF BIOTECHNOLOGY

    ARIHANT PUBLICATION|Exercise Chapter Practice (Short Answer Type-II Questions) |4 Videos

  • PRINCIPLES AND PROCESSES OF BIOTECHNOLOGY

    ARIHANT PUBLICATION|Exercise Chapter Practice (Very Short Answer Type Questions) (Correct the statements, if required, by changing the underlined word(s))|5 Videos

  • ORGANISMS AND ENVIRONMENT

    ARIHANT PUBLICATION|Exercise CHAPTER PRACTICE ( VERY SHORT ANSWER TYPE QUESTIONS ) Short Answer Type-II Question|1 Videos

  • REPRODUCTION IN ORGANISMS

    ARIHANT PUBLICATION|Exercise Chapter Practice ( Short Answer Type-II ) |2 Videos

Similar Questions

Explore conceptually related problems

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Which one of the following characteristics is generally not preferred for a cloning vector ? a) An origin of replication b) An antibiotic resistance marker c)Multiple restriction sites d) A high copy number

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

Match the scientists in column I with their related discoveries in column II and select the correct option from the given codes. {:(ColumnI, Column II),(A."Kary Mullis",(i)"Father of genetic engineering"),(B."Paul Berg",(ii)"Nobel prize for the discovery of restriction endonucleases"),(C."Stanley Cohen and Herbert Boyer",(iii)"Developed polymerase chain reaction"),(D."Arber smith and Nathan",(iv)"Isolated an antibiotic resistance gene from a plasmid of the bacterium Salmonella typhimurium"):}

If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grown and the untransformed recipient cells will die. The ampicillin resistant gene in this case is called as 1) selectable marker 2) recombinant protein 3) cloning site 4) chemical scalpels

Electroporation procedure involves :

Differentiate between Transformation and screening.

ARIHANT PUBLICATION-PRINCIPLES AND PROCESSES OF BIOTECHNOLOGY -Chapter Practice (Very Short Answer Type Questions) (Express in one or two senstence(s))
  1. Define elution.

    Text Solution

    |

  2. The screening procedure for transformants involving antibiotic resista...

    Text Solution

    |