Sterlisation by autoclaving is carried out to

Options

A. Kill bacteria and other pathogens
B. Kill viruses
C. Kill bacteria and enzymes
D. Inactivate enzymes

Answer

**Step-by-Step Solution:**

1. **Understanding Sterilization**:
- Sterilization is the process of making something free from bacteria or other living microorganisms. In laboratory settings, it is crucial to sterilize surfaces, instruments, and media to prevent contamination that could affect experimental results.

2. **Methods of Sterilization**:
- There are various methods of sterilization, including UV sterilization, chemical disinfectants, and autoclaving. Each method has its own applications and effectiveness.

3. **What is Autoclaving?**:
- Autoclaving is a common sterilization method that uses pressurized steam. It typically operates at a temperature of 121 degrees Celsius for a duration of 15 to 20 minutes.

4. **Effectiveness of Autoclaving**:
- The high temperature and pressure during autoclaving are effective in killing bacteria, bacterial spores, and other pathogens. This makes it a reliable method for sterilizing laboratory equipment and media.

5. **Analyzing the Options**:
- **Option A**: Kill bacteria and other pathogens - This is correct as autoclaving effectively kills a wide range of microorganisms.
- **Option B**: Kill viruses - This is incorrect because viruses are non-living entities and do not require sterilization in the same way as living organisms.
- **Option C**: Kill bacteria and enzymes - This is misleading; while autoclaving does kill bacteria, it also denatures enzymes due to high temperatures, making this option incorrect.
- **Option D**: Inactivate enzymes - This is also incorrect as it implies that enzymes can be sterilized, but in reality, they lose their functionality at high temperatures.

6. **Conclusion**:
- Based on the analysis, the correct answer is **Option A: Kill bacteria and other pathogens**.

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