In bacterial/tissue culture, glassware and nutrients are streilised through

Options

A. Water bath at `200^(@)`C
B. Dry air oven at `200^(@)C`
C. Dehumidifier
D. Autoclave at `200^(@)`C

Answer

**Step-by-Step Solution:**

1. **Understanding the Context**: In bacterial and tissue culture, it is crucial to maintain a sterile environment to prevent contamination from unwanted microorganisms. This is essential for the success of the culture.

2. **Identifying the Sterilization Method**: The question asks about the method used to sterilize glassware and nutrients in bacterial or tissue culture. The most common and effective method for sterilization in laboratory settings is the use of an autoclave.

3. **Function of the Autoclave**: An autoclave sterilizes equipment and media by using high-pressure steam. It operates similarly to a pressure cooker, where steam is generated under high pressure, effectively killing microorganisms.

4. **Sterilization Conditions**: The autoclave typically operates at a temperature of 121 degrees Celsius for 15 to 20 minutes. This temperature and duration are sufficient to eliminate bacteria, viruses, and spores.

5. **Preparation for Sterilization**: Before placing the glassware and media in the autoclave, they are washed with detergent to remove any residues. The glassware is then wrapped in newspaper or other suitable materials to maintain sterility during the process.

6. **Final Confirmation**: After the sterilization cycle is complete, the glassware and nutrients are considered sterile and can be used for bacterial or tissue culture without the risk of contamination.

**Conclusion**: The correct answer to the question is that glassware and nutrients in bacterial/tissue culture are sterilized using an autoclave.

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