Match the following pairs correctly and choose the right combination
`{:(,"Column-I",,"Column-II"),(A.,"Escherichia coil",1.,"'nif' gene"),(B.,"Rhizobium melilotae",2.,"Digests hydrocarbon of"),(,,,"crude oil"),(C.,"Bacillus thurigiensis",3.,"Production of human insulin"),(D.,"Pseudomonas putida",4.,"Biological control of fungal"),(,,,"disease"),(,,5.,"Bio-decomposed inserctiside"):}`

Options

A. (a)-(iii),(b)-(i),(c )-(v),(d)-(iv)
B. (a)-(i),(b)-(ii),( c)-(iii),(d)-(iv)
C. (a)-(iii),(b)-(i),(c )-(v),(d)-(ii)
D. (a)-(ii),(b)-(i),(c )-(iii),(d)-(iv)

Answer

To solve the problem of matching the organisms in Column I with their respective functions or characteristics in Column II, we will analyze each organism and its corresponding function step by step.

### Step-by-Step Solution:

1. **Identify Organism A: Escherichia coli (E. coli)**
- E. coli is commonly used in genetic engineering for the production of human insulin.
- **Match:** A (Escherichia coli) → 3 (Production of human insulin)

2. **Identify Organism B: Rhizobium meliloti**
- Rhizobium meliloti is known for its role in nitrogen fixation and contains the 'nif' gene, which is essential for this process.
- **Match:** B (Rhizobium meliloti) → 1 ('nif' gene)

3. **Identify Organism C: Bacillus thuringiensis**
- Bacillus thuringiensis is a gram-positive bacterium that is used as a biodegradable insecticide. It produces a protein crystal toxic to insects.
- **Match:** C (Bacillus thuringiensis) → 5 (Bio-decomposed insecticide)

4. **Identify Organism D: Pseudomonas putida**
- Pseudomonas putida is known for its ability to digest hydrocarbons, particularly those found in crude oil, making it useful for bioremediation.
- **Match:** D (Pseudomonas putida) → 2 (Digests hydrocarbon of crude oil)

### Final Matches:
- A → 3 (Escherichia coli → Production of human insulin)
- B → 1 (Rhizobium meliloti → 'nif' gene)
- C → 5 (Bacillus thuringiensis → Bio-decomposed insecticide)
- D → 2 (Pseudomonas putida → Digests hydrocarbon of crude oil)

### Conclusion:
The correct combination of matches is:
- A - 3
- B - 1
- C - 5
- D - 2

Thus, the correct answer is **Option 3**.