Which of the following cannot be grown on artificial culture medium?

Options

A. Escherichia coli
B. TMV
C. Aspergillus
D. Yeast.

Answer

To determine which of the following organisms cannot be grown on artificial culture medium, we need to analyze each option based on their metabolic capabilities.

1. **Identify the Organisms**: The options given are:
- Sertia coli (a bacterium)
- TMV (Tobacco Mosaic Virus)
- Aspergillus (a fungus)
- Yeast (a fungus)

2. **Understand Metabolism**: Organisms that can be grown on artificial culture medium must possess their own metabolism. This means they can carry out the necessary biochemical reactions independently.

3. **Analyze Each Option**:
- **Sertia coli**: This is a bacterium. Bacteria have their own metabolism and can be cultured on artificial media. Therefore, it can be grown on artificial culture medium.
- **TMV (Tobacco Mosaic Virus)**: This is a virus. Viruses do not have their own metabolism; they require a host cell to replicate and carry out metabolic processes. Thus, TMV cannot be grown on artificial culture medium.
- **Aspergillus**: This is a fungus. Fungi have their own metabolic processes and can be cultured on artificial media. Therefore, it can be grown on artificial culture medium.
- **Yeast**: Yeast is also a fungus and possesses its own metabolism. It can be grown on artificial culture medium.

4. **Conclusion**: Based on the analysis, the organism that cannot be grown on artificial culture medium is **TMV (Tobacco Mosaic Virus)** because it lacks its own metabolic processes and relies on a host cell for replication.

### Final Answer:
**TMV (Tobacco Mosaic Virus)** cannot be grown on artificial culture medium.

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