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A seed cut into two halves and immersed ...

A seed cut into two halves and immersed in `0.1 %` triphenyl tetrazolium chloride solution . Its viability is indicated by development of colouration

A

Yellow

B

Black

C

Blue

D

Red

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To determine the viability of a seed using triphenyl tetrazolium chloride (TTC), follow these steps: ### Step-by-Step Solution: 1. **Preparation of the Seed**: - Take a seed and soak it in water overnight. This helps to soften the seed coat and prepares it for the next step. 2. **Cutting the Seed**: - After soaking, carefully cut the seed into two halves. This is done to expose the embryo, which is the living part of the seed. 3. **Immersion in TTC Solution**: - Immerse the two halves of the seed in a 0.1% solution of triphenyl tetrazolium chloride (TTC). This solution is used to test the viability of the seed. 4. **Observation of Color Change**: - After a certain period of time (usually a few hours), observe the color of the seed halves. If the seed is viable, the embryo will react with the TTC. 5. **Interpretation of Results**: - The development of a red coloration indicates that the seed is viable. This red color is due to the formation of formazine, a reddish compound formed when dehydrogenase enzymes present in the living tissue of the embryo react with the TTC. 6. **Conclusion**: - If the seed shows a red color, it confirms that the seed is viable. If there is no color change or a different color appears (like yellow, black, or blue), it indicates that the seed is not viable. ### Final Answer: The viability of the seed is indicated by the development of the red coloration when immersed in 0.1% triphenyl tetrazolium chloride solution. ---
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