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Substrate concentration at which an enzy...

Substrate concentration at which an enzyme attains half its maximum velocity is

A

Threshold value

B

Half-life

C

Michaelis-Menten constant

D

Concentration coefficient.

Text Solution

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The correct Answer is:
To solve the question regarding the substrate concentration at which an enzyme attains half its maximum velocity, we can follow these steps: ### Step-by-Step Solution: 1. **Understand the Question**: The question asks for the substrate concentration at which an enzyme reaches half of its maximum velocity (Vmax). 2. **Define Key Terms**: - **Enzyme**: A biological catalyst that speeds up chemical reactions. - **Vmax**: The maximum rate of an enzyme-catalyzed reaction when the enzyme is saturated with substrate. - **Michaelis-Menten Constant (KM)**: A key parameter in enzyme kinetics that represents the substrate concentration at which the reaction velocity is half of Vmax. 3. **Relate Vmax and KM**: According to the Michaelis-Menten equation, when the substrate concentration is equal to KM, the reaction velocity (V) is half of Vmax. This means that at this concentration, half of the enzyme active sites are occupied by the substrate. 4. **Evaluate the Options**: - **Threshold Value**: Incorrect, as it refers to a minimum value for a reaction to occur. - **Half-Life**: Incorrect, as it relates to the time taken for a substance to reduce to half its initial quantity. - **Concentration Coefficient**: Incorrect, as it does not pertain to enzyme kinetics. - **Michaelis-Menten Constant (KM)**: Correct, as it is defined as the substrate concentration at which the reaction velocity is half of Vmax. 5. **Conclusion**: The substrate concentration at which an enzyme attains half its maximum velocity is the Michaelis-Menten constant, denoted as KM. ### Final Answer: The correct answer is **Michaelis-Menten constant (KM)**. ---
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