Specificity of protein in enzyme action depends upon

Options

A. (a) Active sites
B. (b) Linear sequence of amino acids
C. (c) `K_(m)` constant
D. (d) Turn over number

Explanation

To answer the question regarding the specificity of protein in enzyme action, we can break down the reasoning step by step:

### Step-by-Step Solution:

1. **Understanding Enzymes and Proteins**:
- Enzymes are proteins that act as catalysts in biochemical reactions. They speed up reactions without being consumed in the process.

2. **Role of Amino Acids**:
- Proteins are made up of long chains of amino acids. The sequence and composition of these amino acids determine the structure and function of the protein.

3. **Linear Sequence of Amino Acids**:
- The specificity of an enzyme is largely determined by its unique linear sequence of amino acids. This sequence dictates how the protein folds into its three-dimensional structure, which is crucial for its function.

4. **Active Sites**:
- While enzymes have active sites where substrates bind, the specificity of the enzyme is primarily determined by the linear sequence of amino acids that forms the active site. Therefore, while the active site is important, it is the sequence that defines its characteristics.

5. **Other Options**:
- The KM constant and turnover number are parameters that describe enzyme kinetics, but they do not determine the specificity of the enzyme. They are influenced by the enzyme's structure but do not define it.

6. **Conclusion**:
- Based on the above reasoning, the specificity of protein in enzyme action depends on the linear sequence of amino acids (Option B).

### Final Answer:
The specificity of protein in enzyme action depends upon the **linear sequence of amino acids** (Option B).

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