Q. Golgi was able to differentiate Golgi apparatus through

Options

A. a) Phase contrast microscopy
B. b) Metallic impregnation technique
C. c) Electron microscopy
D. d) Special redox dye.

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks which method Golgi used to differentiate the Golgi apparatus.

2. **Identifying the Options**: The options provided are:
- A. Phase contrast microscopy
- B. Metallic impermeation technique
- C. Electron microscopy
- D. Special redox dye

3. **Knowledge of Golgi Apparatus**: The Golgi apparatus is an essential organelle in the cell responsible for modifying, sorting, and packaging proteins and lipids for secretion or delivery to other organelles.

4. **Historical Context**: The Golgi apparatus was discovered by the scientist Camillo Golgi. Understanding the historical context is important to identify the correct method he used.

5. **Evaluating the Methods**:
- **Phase Contrast Microscopy**: This is a technique used to enhance the contrast in transparent and colorless specimens. It was not the method used by Golgi.
- **Metallic Impermeation Technique**: This technique involves using heavy metals to stain cellular structures, making them visible under a microscope. This is the method used by Golgi to identify the Golgi apparatus.
- **Electron Microscopy**: While this method provides high-resolution images of cellular structures, it was developed after Golgi's time.
- **Special Redox Dye**: This method is not specifically associated with the discovery of the Golgi apparatus.

6. **Conclusion**: Based on the evaluation of the methods, the correct answer is B. Metallic impermeation technique, as it was the method used by Golgi to differentiate the Golgi apparatus.

**Final Answer**: B. Metallic impermeation technique

---