Protoplast does not include

Options

A. Plasma membrane
B. Cell wall
C. Nucleus
D. Cytoplasm

Answer

To answer the question "Protoplast does not include," we need to understand what a protoplast is and what components it contains.

### Step-by-Step Solution:

1. **Define Protoplast**:
- A protoplast is a living cell that has had its cell wall removed. It consists of the plasma membrane and the contents within it, including the cytoplasm and the nucleus.

2. **Identify Components of Protoplast**:
- The protoplast includes:
- **Plasma Membrane**: This is essential as it surrounds the protoplast and maintains the integrity of the cell.
- **Cytoplasm**: This is the gel-like substance within the cell where various organelles are suspended.
- **Nucleus**: This is the control center of the cell that contains genetic material.

3. **Determine What is Not Included**:
- The key point is that the protoplast does not include the **cell wall**. The cell wall is a rigid outer layer found in plant cells, fungi, and some bacteria, but it is not part of the protoplast.

4. **Evaluate the Options**:
- Given the options:
- A) Plasma Membrane - **Included**
- B) Cell Wall - **Not Included** (Correct Answer)
- C) Nucleus - **Included**
- D) Cytoplasm - **Included**

5. **Conclusion**:
- The correct answer to the question "Protoplast does not include" is **B) Cell Wall**.