Puffs of polytene chromosome are specially concentrated with

Options

A. DNA polymerase
B. Ligase
C. Ecdysone
D. RNA polymerase

Answer

To solve the question regarding what puffs of polytene chromosomes are especially concentrated with, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Polytene Chromosomes**:
- Polytene chromosomes are large chromosomes that have undergone multiple rounds of DNA replication without cell division, resulting in many identical strands of DNA. They are typically found in certain tissues of organisms like Drosophila (fruit flies).

2. **Identifying Puffs**:
- The "puffs" on polytene chromosomes refer to regions where the chromatin is less condensed and appears swollen. These regions are indicative of active transcription.

3. **Function of Puffs**:
- The puffs are sites of gene expression where RNA synthesis occurs. This means that the DNA in these regions is being transcribed into RNA.

4. **Enzymes Involved in RNA Synthesis**:
- The primary enzyme responsible for synthesizing RNA from a DNA template is RNA polymerase. This enzyme binds to the DNA at the promoter region and catalyzes the formation of RNA.

5. **Analyzing the Options**:
- The options given are DNA polymerase, ligase, egg dyson, and RNA polymerase.
- DNA polymerase is involved in DNA replication, not RNA synthesis.
- Ligase is involved in joining DNA fragments together.
- Egg dyson is not a relevant enzyme in this context.
- RNA polymerase is the enzyme responsible for RNA synthesis.

6. **Conclusion**:
- Since puffs of polytene chromosomes are sites of active RNA synthesis, they are especially concentrated with RNA polymerase.

### Final Answer:
The correct answer is **RNA polymerase**.

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