Hirudin gene inserted in Brassica napus was

Options

A. Synthesised chemically
B. Obtained from leech
C. Got from Bacillus thuringiensis
D. Brassica campestris.

Answer

### Step-by-Step Solution:

1. **Understanding the Hirudin Gene**:
- Hirudin is a protein that acts as an anticoagulant, preventing blood clotting. It is derived from the saliva of leeches, specifically from the species Hirudo medicinalis.

2. **Source of the Gene**:
- The gene coding for Hirudin is obtained from leeches. This gene can be chemically synthesized or cloned from leech DNA.

3. **Insertion into Brassica napus**:
- The Hirudin gene is inserted into the genome of Brassica napus, a species of rapeseed, using biotechnological methods such as Agrobacterium-mediated transformation or gene gun techniques.

4. **Accumulation in Seeds**:
- Once the gene is successfully integrated, the Brassica napus plants express the Hirudin protein, which accumulates in the seeds of the plant.

5. **Extraction and Purification**:
- The seeds are harvested, and the Hirudin protein is extracted from them. This involves breaking down the seed tissues and isolating the protein.

6. **Medicinal Use**:
- The extracted Hirudin is then purified to remove any impurities and is prepared for medical applications, primarily as an anticoagulant in various therapeutic contexts.

7. **Conclusion**:
- The correct answer to the question is that the Hirudin gene inserted in Brassica napus is used to produce Hirudin, which is then extracted, purified, and used for medicinal purposes.