During gene amplification the mixture of sample DNA, nucleotides and polymerases is heated to

Options

A. Activate polymerses
B. Activate nucleotides
C. Separates sense and antisense DNA strands
D. Provides energy for different strands.

Answer

**Step-by-Step Solution:**

1. **Understanding Gene Amplification**: Gene amplification is a process used to replicate a specific segment of DNA. This is commonly achieved through a technique called Polymerase Chain Reaction (PCR).

2. **Components of the Reaction**: The mixture for gene amplification typically includes sample DNA, nucleotides (the building blocks of DNA), and DNA polymerases (enzymes that synthesize new DNA strands).

3. **Heating the Mixture**: The first step in the PCR process is called denaturation. During this step, the mixture is heated to separate the two strands of the DNA helix.

4. **Temperature for Denaturation**: The temperature required for denaturation is typically between 94 to 95 degrees Celsius. At this temperature, the hydrogen bonds between the DNA strands break, allowing the strands to separate.

5. **Conclusion**: Therefore, during gene amplification, the mixture of sample DNA, nucleotides, and polymerases is heated to **94 to 95 degrees Celsius** to achieve denaturation.

**Final Answer**: The mixture is heated to **94 to 95 degrees Celsius**.

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