A major use of embryo culture is in

Options

A. induction of somaclonal variations
B. Overcoming hybridisation barriers
C. Production of alkaloids
D. Clonal propagation.

Answer

**Step-by-Step Solution:**

1. **Understanding Embryo Culture**: Begin by defining what embryo culture is. It is a technique used in plant breeding that involves the cultivation of embryos in vitro (outside the organism) to facilitate the development of plants.

2. **Recognizing the Context of Embryo Culture**: Understand that embryo culture is often used in the context of sexual reproduction in plants, where fertilization occurs between male and female gametes, leading to the formation of a zygote and subsequently an embryo.

3. **Identifying the Purpose of Embryo Culture**: One of the major uses of embryo culture is to overcome hybridization barriers. Hybridization barriers can prevent successful fertilization and development between different species or varieties.

4. **Evaluating Other Options**:
- **Induction of Somaclonal Variations**: This refers to variations that occur in plants regenerated from tissue culture, which is not the primary use of embryo culture.
- **Production of Alkaloids**: This is a process related to secondary metabolites and not directly linked to embryo culture.
- **Clonal Propagation**: While related to plant propagation, it is distinct from the specific application of embryo culture.

5. **Conclusion**: After evaluating the options, the major use of embryo culture is confirmed to be in overcoming hybridization barriers.

**Final Answer**: The major use of embryo culture is in overcoming hybridization barriers.

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