Axenic culture is
(a) pure culture without any contamination
(b) pure culture without any nutrient
(c) Culture of tissue
(d) Culture of gene

Options

A. pure culture without any contaminaion
B. pure culture without any nutrient
C. Culture of tissue
D. Culture of gene

Answer

To solve the question regarding axenic culture, we will analyze each option provided and determine which one accurately describes axenic culture.

### Step-by-Step Solution:

1. **Understanding the Term "Axenic Culture":**
- Axenic culture refers to a culture that is free from any other living organisms except for the specific microorganism being studied. This means it is a pure culture.

2. **Analyzing Option (a): "pure culture without any contamination":**
- This option states that axenic culture is a pure culture without contamination. Since axenic cultures are indeed free from other organisms, this option aligns with the definition of axenic culture.

3. **Analyzing Option (b): "pure culture without any nutrient":**
- This option suggests that axenic culture lacks nutrients. However, axenic cultures do contain nutrients necessary for the growth of the specific microorganism being cultured. Therefore, this option is incorrect.

4. **Analyzing Option (c): "Culture of tissue":**
- This option describes a tissue culture, which is different from axenic culture. Tissue culture involves growing cells or tissues in a controlled environment, but it does not specifically refer to the absence of contamination. Thus, this option is not correct.

5. **Analyzing Option (d): "Culture of gene":**
- This option refers to genetic material or gene culture, which is not relevant to the definition of axenic culture. Axenic culture specifically pertains to microorganisms, not genes. Therefore, this option is also incorrect.

6. **Conclusion:**
- Based on the analysis, the correct answer is option (a): "pure culture without any contamination."

### Final Answer:
(a) pure culture without any contamination

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