The technique that was employed to produce haploids of datura was :

Options

A. Meristem culture
B. Anther culture
C. Embryo culture
D. Protoplast culture

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks for the specific technique used to produce haploids of the Datura plant.

2. **Identifying the Techniques**: The options provided are:
- Meristem culture
- Anther culture
- Embryo culture
- Protoplast culture

3. **Analyzing Each Technique**:
- **Meristem Culture**: This involves culturing the meristematic tissue, usually apical meristem, which typically produces diploid plants, not haploids.
- **Anther Culture**: This technique involves culturing the anther, which contains haploid microspore cells. This method is specifically used to produce haploid plants, known as androgenic haploids.
- **Embryo Culture**: This technique uses immature embryos to grow new plants, which also does not produce haploids.
- **Protoplast Culture**: This involves the culture of protoplasts (cells without cell walls), which can be used to regenerate plants, but again does not specifically produce haploids.

4. **Conclusion**: After analyzing the techniques, it is clear that the correct answer is **Anther Culture**, as it is the method specifically used to produce haploids of Datura.

5. **Final Answer**: The technique that was employed to produce haploids of Datura is **Anther Culture**.

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