Pollen grains of a plant whose 2n = 28 are cultured to get callus by tissue culture method. What would be the number of chromosomes in the cells of the callus ?

Options

A. 56
B. 28
C. 21
D. 14

Answer

To solve the question regarding the number of chromosomes in the cells of the callus derived from pollen grains of a plant with a diploid number (2n) of 28, follow these steps:

### Step-by-Step Solution:

1. **Identify the Diploid Number (2n)**:
- The given diploid number (2n) of the plant is 28. This means that the plant has a total of 28 chromosomes in its somatic (body) cells.

2. **Understand the Pollen Grain Chromosome Number**:
- Pollen grains are produced through the process of meiosis, which reduces the chromosome number by half. Therefore, pollen grains are haploid (n).

3. **Calculate the Haploid Number (n)**:
- To find the haploid number (n), divide the diploid number (2n) by 2:
\[ n = \frac{2n}{2} = \frac{28}{2} = 14 \]
- Thus, the pollen grains contain 14 chromosomes.

4. **Callus Formation**:
- When pollen grains are cultured to form a callus, the callus cells will also have the same chromosome number as the pollen grains because they are derived from them.

5. **Conclusion**:
- Therefore, the number of chromosomes in the cells of the callus will be 14.

### Final Answer:
The number of chromosomes in the cells of the callus is **14**.

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