The totipotent cells form a

Options

A. Bud
B. Cell membrane
C. Cell organelle
D. Complete new organism

Answer

### Step-by-Step Solution:

1. **Understanding Totipotency**:
- Totipotent cells are unique in that they have the ability to develop into a complete organism. This means they can differentiate into any type of cell needed for the formation of a new organism.

2. **Definition of Totipotent Cells**:
- These cells are typically found in the early stages of embryonic development. In plants, totipotent cells can arise from somatic cells or explants, which are parts of the plant used for tissue culture.

3. **Tissue Culture Process**:
- In plant tissue culture, explants are treated with antimicrobial chemicals to eliminate any potential microbial contamination. This is crucial for the successful growth of the plant cells in a controlled environment.

4. **Preparation of Culture Media**:
- The culture media used for growing these cells contains essential nutrients, including inorganic salts, vitamins, sucrose (a source of energy), and specific growth regulators that promote cell division and differentiation.

5. **Growth Medium Types**:
- The growth medium can be liquid, solid, or semi-solid (like agar). This medium provides the necessary environment for the totipotent cells to grow and develop into a complete organism.

6. **Conclusion**:
- Therefore, the correct answer to the question is that totipotent cells form a "complete new organism."

### Final Answer:
Totipotent cells form a complete new organism.

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