Monoclonal antibodies are produced from hybrid cells, called hybridomas. The cells employed to obtain these hybridoma cells are:

Options

A. T-lymphocytes and myeloma cells
B. B-lymphocytes and myeloma cells
C. B-lymphocytes and myeloma cells
D. Lymphoma cells bone marrow cells

Answer

To answer the question about the cells employed to obtain hybridoma cells for the production of monoclonal antibodies, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Hybridomas**: Hybridomas are hybrid cells that are formed by the fusion of two different types of cells. This technology is used to produce monoclonal antibodies, which are antibodies that are identical and specific to a particular antigen.

2. **Identifying the Cells Involved**: The two types of cells that are fused to create hybridomas are:
- **B lymphocytes (B cells)**: These are a type of white blood cell that produces antibodies.
- **Myeloma cells**: These are cancerous cells that can grow indefinitely in culture and do not produce antibodies.

3. **Fusion Process**: The process of creating hybridomas involves fusing B lymphocytes, which are taken from an immunized animal (often a mouse), with myeloma cells. This fusion allows the resulting hybridoma to have the ability to produce a specific antibody (from the B cell) while also being able to proliferate indefinitely (from the myeloma cell).

4. **Selection of Hybridomas**: After fusion, the hybrid cells are selected based on their ability to grow in tissue culture and their capacity to produce the desired antibody. The hybridomas that successfully produce the specific antibody are then cloned to produce large quantities of monoclonal antibodies.

5. **Conclusion**: Based on the above information, the correct answer to the question is that the cells employed to obtain hybridoma cells are **B lymphocytes and myeloma cells**.

### Final Answer:
The cells employed to obtain hybridoma cells are **B lymphocytes and myeloma cells**.