Mention the names of biological tools used in recombinant DNA technology.

The first step in recombinant DNA technology is

What is recombinant DNA ?

Name some biological agents.

Mention the biological agents playing significant role in biotechnology.

..... enzymes are used as biological scissors in r- DNA technology.

The.... most commonly used in recombinant DNA technology are those that replicate in E. coli.

Genetic engineering is alternatively called as Recombinant DNA technology or Gene Cloning: Give scientific reason.

Give the examples of competent host used in rDNA technology.

Name the biological tools for rDNA technology.

Rewrite the following paragraph by writting most appropriate words in the blank places. Insulin is a....... hormone produced by beta cells of islets of Langerhans of pancreas, insulin is essential for the control of blood sugar levels......... is a disease in which some people cannot make insulin themselves. Hakura et al, chemically synthesized DNA sequence of insulin for two chains....... and separately inserted into two....... vector. Insulin production by recombinant DNA technology is designed by Gilbert and Villa -Komaroff. The genes are inserted by the side of ..... gene of the plasmid. The recombinant plasmids were then separately transformed into E. coil host. The host produced penicillinase + pre-pro insulin . Insulin is later separated by ......... treatment.