To produce insulin on industrial scale, human gene for insulin is isolated and cloned in a host bacterium . Then the final product is extracted and purified . Explain in detail.

Diseases in humans can be prevented by administration of vaccines. Vaccines contain inactivated, killed / weakened pathogens that can be used as antigens to provide either permanent or temporary immunity against a specific pathogen or disease. Conventional methods of vaccine preparation include the use of completely or partially killed pathogens. These vaccines however posed a risk of causing disease in case of some persons. Biotechnological techniques provide alternative antigenic genes isolated in the laboratory that can be used to make pure forms of proteins that can be used as antigens to activate the immune system. Recent developments in this field include production of edible vaccines like transgenic potatoes. Consumption of raw potatoes is speculated to develop immunity against the pathogen. These edible vaccines are produced by inserting genes isolated from specific pathogens to produce the antigen in the plants. Based on the given paragraph answer the following questions. Why are vaccines produced using biotechnology safer than conventional vaccines?

Diseases in humans can be prevented by administration of vaccines. Vaccines contain inactivated, killed / weakened pathogens that can be used as antigens to provide either permanent or temporary immunity against a specific pathogen or disease. Conventional methods of vaccine preparation include the use of completely or partially killed pathogens. These vaccines however posed a risk of causing disease in case of some persons. Biotechnological techniques provide alternative antigenic genes isolated in the laboratory that can be used to make pure forms of proteins that can be used as antigens to activate the immune system. Recent developments in this field include production of edible vaccines like transgenic potatoes. Consumption of raw potatoes is speculated to develop immunity against the pathogen. These edible vaccines are produced by inserting genes isolated from specific pathogens to produce the antigen in the plants. Based on the given paragraph answer the following questions. What is the disadvantage of transgenic potatoes containing edible vaccines?

Diseases in humans can be prevented by administration of vaccines. Vaccines contain inactivated, killed / weakened pathogens that can be used as antigens to provide either permanent or temporary immunity against a specific pathogen or disease. Conventional methods of vaccine preparation include the use of completely or partially killed pathogens. These vaccines however posed a risk of causing disease in case of some persons. Biotechnological techniques provide alternative antigenic genes isolated in the laboratory that can be used to make pure forms of proteins that can be used as antigens to activate the immune system. Recent developments in this field include production of edible vaccines like transgenic potatoes. Consumption of raw potatoes is speculated to develop immunity against the pathogen. These edible vaccines are produced by inserting genes isolated from specific pathogens to produce the antigen in the plants. Based on the given paragraph answer the following questions. What is the main advantage of vaccines over antibiotics?

Diseases in humans can be prevented by administration of vaccines. Vaccines contain inactivated, killed / weakened pathogens that can be used as antigens to provide either permanent or temporary immunity against a specific pathogen or disease. Conventional methods of vaccine preparation include the use of completely or partially killed pathogens. These vaccines however posed a risk of causing disease in case of some persons. Biotechnological techniques provide alternative antigenic genes isolated in the laboratory that can be used to make pure forms of proteins that can be used as antigens to activate the immune system. Recent developments in this field include production of edible vaccines like transgenic potatoes. Consumption of raw potatoes is speculated to develop immunity against the pathogen. These edible vaccines are produced by inserting genes isolated from specific pathogens to produce the antigen in the plants. Based on the given paragraph answer the following questions. i) How can people immunized with vaccines containing semi-killed pathogens have a risk of contracting a disease?

Diseases in humans can be prevented by administration of vaccines. Vaccines contain inactivated, killed / weakened pathogens that can be used as antigens to provide either permanent or temporary immunity against a specific pathogen or disease. Conventional methods of vaccine preparation include the use of completely or partially killed pathogens. These vaccines however posed a risk of causing disease in case of some persons. Biotechnological techniques provide alternative antigenic genes isolated in the laboratory that can be used to make pure forms of proteins that can be used as antigens to activate the immune system. Recent developments in this field include production of edible vaccines like transgenic potatoes. Consumption of raw potatoes is speculated to develop immunity against the pathogen. These edible vaccines are produced by inserting genes isolated from specific pathogens to produce the antigen in the plants. Based on the given paragraph answer the following questions. How does the immune system react on administration of vaccines?

Diagrammatically represent the experimental steps in cloning and expressing a human gene ( say the gene foe insulin)into a bacterium like E. coli.

Rewrite the following paragraph by writting most appropriate words in the blank places. Insulin is a....... hormone produced by beta cells of islets of Langerhans of pancreas, insulin is essential for the control of blood sugar levels......... is a disease in which some people cannot make insulin themselves. Hakura et al, chemically synthesized DNA sequence of insulin for two chains....... and separately inserted into two....... vector. Insulin production by recombinant DNA technology is designed by Gilbert and Villa -Komaroff. The genes are inserted by the side of ..... gene of the plasmid. The recombinant plasmids were then separately transformed into E. coil host. The host produced penicillinase + pre-pro insulin . Insulin is later separated by ......... treatment.